Antiperoxidative properties of oil mixes of high ratio Omega 9 Omega 6 and low ratio Omega 6 Omega 3 after molar extraction in rats

January 1, 2014 Human Health and Nutrition Data 0 Comments

Antiperoxidative properties of oil mixes of high ratio Omega 9 Omega 6 and low ratio Omega 6 Omega 3 after molar extraction in rats

Year: 2014
Authors: Melol, R.B. Guimaraesll, S.B. de Barros Silvalll, P.G. OriaIV, R.B. de Souza MeloV, J.U. Leitao, P.R.
Publication Name: Acta Cir Bras
Publication Details: Volume 29; Issue 6; pages 371-5.

Abstract:

To evaluate the antioxidant and anti peroxidative effects of oil mixes of high ratio Omega 9 Omega 6 and low ratio Omega 6 Omega 3 in the third day after tooth extraction in rats. Thirty two male Wistar rats (270 to 310g) were randomly distributed in two groups  Control and Test. Control group was divided into three subgroups  G1  Sham Saline; G2  Saline; G3  Isolipid. G1 and G2 animals received NaCl 0.9 per cent while G3 rats were treated with an isolipid mixture (alpha linolenic acid   ALA) containing   6 per  3 oils and  9 per  6. Test group animals (G4) received oily mixtures (alpha linolenic acid  ALA, docosahexaenoic acid   DHA, eicosapentaenoic acid  EPA) of  6 per  3 and   9 per  6 . Saline and oils were administered by gavage during four days before and three days after first mandibular molar extraction. Following, samples (arterial blood and alveolar mucosa) were collected for glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) assays. Oil mixes induced a significant decrease in GSH and TBARS tissue and plasma concentrations in the third day post surgery. Gavage administration of oil mixes of high ratio Omega 9 Omega 6 and low ratio Omega 6 Omega 3 after molar extraction in rats induces a significant decrease in lipid peroxidation. (Authors abstract)
Oxidative stress can be defined as imbalance between production and collection of reactive oxygen species (ROS). The oral supplementation of antioxidants is indicated in several situations, such as cancer, sepsis, preoperative of major surgeries and malnourished or critically ill patients. Omega 3 is one of the most used in research immunonutrients. Protective effects of oils mixes against ischemia per reperfusion injury have been demonstrated. and investigated the effects of preconditioning with mixtures of oils containing high and low ratios of n6 to n3 and n9 to n6. The data found in the literature vary widely as to the best combination of antioxidants, the dosage, route of administration, the levels to be ideally achieved, the best time and how long they have to be administered in order to provide an effective protection against the oxidative injury. The hypothesis of this study is that the use of combinations containing different proportions of omega 3, 6 and 9 amino acids may have a noticeable antioxidant effect during the inflammatory phase of the wound healing after dental extraction. Local and systemic factors may affect rat socket healing, including salivary gland hypofunction, osteoporosis, osteopetrosis, diabetes mellitus and treatment with calcitonin or cortisone. In this work it was found that oil mixes administered by gavage before and after dental extraction may provide protection from lipid peroxidation in the third postoperative day.
 
In this study, the use of an oil mix containing alpha linolenic acid (ALA), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and a n6 to n3 (1.4 to 1 ratio) and n9 to n3 (3.4 1 ratio) promoted a decrease in GSH levels, showing that GSH becomes “lesser necessary” and, in this way, an antioxidant action. Generally, cells react to oxidative stress with a increase in GSH pool as part of their adaptative answer to the potential oxidative lesion. Therefore, a lower GSH level indicates a less aggressive potential oxidative stress. This study shows the antiperoxidative effects of oil mixes containing ALA, DHA, in various ratios when administered by gavage before and after rat tooth extraction. (Editors comments)



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