In vivo 13C NMR determines metabolic fluxes and steady state in linseed embryos

January 1, 2007 Human Health and Nutrition Data 0 Comments

In vivo 13C NMR determines metabolic fluxes and steady state in linseed embryos

Year: 2007
Authors: Troufflard, S., Roscher, A., Thomasset, B., Barbotin, J-N., Rawsthorne, S., Portais, J-C.
Publication Name: Phytochemistry
Publication Details: Volume 68; Pages 2341–2350


The dynamics of developing linseed embryo metabolism was investigated using 13C-labelling experiments where the real-time kinetics of label incorporation into metabolites was monitored in situ using in vivo NMR. The approach took advantage of the occurrence in this plant tissue of large metabolite pools – such as sucrose or lipids – to provide direct and quantitative measurement of the evolution of the labelling state within central metabolism. As a pre-requisite for the use of steady state flux measurements it was shown that isotopic steady state was reached within 3 h at the level of central intermediates whereas it took a further 6 h for the sucrose pool. Complete isotopic and metabolic steady state took 18 h to be reached. The data collected during the transient state where label was equilibrated but the metabolic steady state was incomplete, enabled the rates of lipid and sucrose synthesis to be measured in situ on the same sample. This approach is suitable to get a direct assessment of metabolic time-scales within living plant tissues and provides a valuable complement to steady state flux determinations. Author's Abstract.

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