Linolenic acid prevents neuronal cell death and paraplegia after transient spinal cord ischemia in rats

January 1, 2003 Human Health and Nutrition Data 0 Comments

Linolenic acid prevents neuronal cell death and paraplegia after transient spinal cord ischemia in rats

Year: 2003
Authors: Lang-Lazdunski, L., Blondeau, N., Jarretou, G., Lazdunski, M., Heurteaux, C.
Publication Name: J Vasc Surg
Publication Details: Volume 38; Pages 564-75


Purpose: Spinal cord ischemia is a devastating complication of thoracic and thoracoabdominal aortic surgery. Recent studies have suggested a neuroprotective effect of polyunsaturated fatty acids against cerebral ischemia. We investigated the effect of linolenic acid (LIN) in a rat model of spinal cord ischemia.
Methods: Rats were subjected to cross-clamping of the aortic arch and left subclavian artery for 14 minutes. Groups were as follows: sham operation (n = 15); ischemia (n = 15), receiving only vehicle; LIN A (n = 15), receiving LIN before clamping; and LIN B (n = 15), receiving LIN at onset of reperfusion. Neurologic status was assessed daily for 7 days. Spinal cords were harvested for histopathologic analysis, TUNEL staining, and immunohistochemistry for Bax, heat shock protein 70 (HSP70), and nuclear factor– KB.
Results: Ischemic rats had severe and definitive paraplegia. LIN-treated rats had significantly better neurologic function. Histopathologic analysis disclosed severe neuronal necrosis in the lumbar gray matter of ischemic rats, whereas most of the LIN-treated rats sustained mild to moderate injury. LIN reduced the loss of motor neurons at 7 days (LIN A, 17 ± 6, and LIN B, 15 ± 7, versus ischemia, 6 ± 2 per section; P < .05). LIN prevented apoptotic neuronal cell death, Bax immunoreactivity of the pro-apoptotic protein Bax, and the nuclear transcription factor NF-KB. Nuclear HSP70 immunoreactivity was noted exclusively in motor neurons from LIN-treated rats and not in motor neurons from ischemic rats.
Conclusion: These results suggest that LIN can induce protection against ischemia in the spinal cord, thereby preventing both necrosis and apoptosis of motor neurons. Author's Abstract.

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