Synthesis and Biohydrogenation of Fatty Acids by Ruminal Microorganisms In Vitro.

January 1, 1991 Animal Health and Feed Data 0 Comments

Synthesis and Biohydrogenation of Fatty Acids by Ruminal Microorganisms In Vitro.

Year: 1991
Authors: Wu, Z., Palmquist, D.L.,
Publication Name: J.Dairy Sci.
Publication Details: Volume 74; Pages 3035-3046


Ruminal degradation, synthesis, and biohydrogenation of fatty acids were examined in vitro. Diets were incubated with ruminal contents, and changes of fatty acids were measured. Two fat supplements, a calcium soap and an animalvegetable blend, were included in diets at various levels. Addition to diets of acetate and isoacids (collective term for certain short-chain acids) also were tested for effects on fatty acid synthesis. Overall, 6.6 mg of fatty acids/g of fat-free diet were synthesized during 24-h incubation regardless of supplementations. Fatty acids synthesized in greatest amounts were odd-numbered or branched chains, whereas chains of 16 and 18 carbons changed little, and chains shorter than 14 carbons decreased.  Degradation of [1-14C]palmitic acid was negligible, as determined by recovery of the label in C02 (.03%) and acetate (1.09%) after 4-h incubation with rumen contents. Biohydrogenation of fatty acids averaged 47% in diets containing calcium soap and 71% with animal-vegetable blend. Synthesis and biohydrogenation were similar to those measured previously in vivo, showing that in vitro measurements reliably predicted metabolism of fatty acids in vivo.  Authors Abstract.

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